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ms2 dna encoding a single chain dimer of the ms2 coat protein  (GenScript corporation)

 
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    GenScript corporation ms2 dna encoding a single chain dimer of the ms2 coat protein
    Ms2 Dna Encoding A Single Chain Dimer Of The Ms2 Coat Protein, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ms2+dna+encoding+a+single+chain+dimer+of+the+ms2+coat+protein/dna+encoding+the+single+chain+ms2+coat+protein+dimer/pm39774966-296-4-26
    Average 90 stars, based on 1 article reviews
    ms2 dna encoding a single chain dimer of the ms2 coat protein - by Bioz Stars, 2026-10
    90/100 stars

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    Expressing:

    Article Title: Multivalent S2 subunit vaccines provide broad protection against Clade 1 sarbecoviruses in female mice.
    Article Snippet: Protein concentration was quantified using the bicinchoninic acid assay (BCA) assay (Thermo Scientific). .. Expression and purification of MS2 DNA encoding a single chain dimer of theMS2 coat proteinwas cloned into pET-28b between the NdeI and XhoI restriction sites by GenScript Biotech Corporation (Piscataway, NJ) with the additional insertion of an AviTag between the 14th and 15th residues of the first coat protein monomer2,12,14,24. .. This plasmid was co-transformed with a BirA-biotinprotein ligase expressing plasmid into BL21(DE3) competent E. coli (New England Biolabs) per manufacturer protocol.

    Purification:

    Article Title: Multivalent S2 subunit vaccines provide broad protection against Clade 1 sarbecoviruses in female mice.
    Article Snippet: Protein concentration was quantified using the bicinchoninic acid assay (BCA) assay (Thermo Scientific). .. Expression and purification of MS2 DNA encoding a single chain dimer of theMS2 coat proteinwas cloned into pET-28b between the NdeI and XhoI restriction sites by GenScript Biotech Corporation (Piscataway, NJ) with the additional insertion of an AviTag between the 14th and 15th residues of the first coat protein monomer2,12,14,24. .. This plasmid was co-transformed with a BirA-biotinprotein ligase expressing plasmid into BL21(DE3) competent E. coli (New England Biolabs) per manufacturer protocol.

    Clone Assay:

    Article Title: Multivalent S2 subunit vaccines provide broad protection against Clade 1 sarbecoviruses in female mice.
    Article Snippet: Protein concentration was quantified using the bicinchoninic acid assay (BCA) assay (Thermo Scientific). .. Expression and purification of MS2 DNA encoding a single chain dimer of theMS2 coat proteinwas cloned into pET-28b between the NdeI and XhoI restriction sites by GenScript Biotech Corporation (Piscataway, NJ) with the additional insertion of an AviTag between the 14th and 15th residues of the first coat protein monomer2,12,14,24. .. This plasmid was co-transformed with a BirA-biotinprotein ligase expressing plasmid into BL21(DE3) competent E. coli (New England Biolabs) per manufacturer protocol.



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